Article Sidebar

Submitted
Feb 6, 2020
Published
Apr 28, 2020
Download
Vol 6 No 1
Statistic
Read Counter : 559 Download : 4535

Downloads

Download data is not yet available.

Main Article Content

Abstract

Antioxidants in the form of total flavonoids are compounds that can stabilize free
radicals. The mechanism of antioxidants in stabilizing free radicals is give the lack
electron of free radicals and inhibit the formation of free radical formation that
can damage the body. DPPH method is used to determine the activity of capturing
free radicals of ethanol extracts of Amaranthus tricolor L. red giti and green giti.
As a positive control quercetin is used. The results showed that the total amount of
flavonoids in red giti spinach was (5.615±0.114)% EK and green giti spinach was
(4.468±0.166)% EK. The results also show that all groups have activity as free
radical scavengers. The value of ES 50 for positive control quercetin, red giti
spinach extract, and green giti spinach extract are (1.375±0.085)µg/ml,
(186.198±0.363)µg/ml, and (209.392± 0.607)µg/ml respectively. Based on ES 50
values It can be concluded that antioxidant activity of red giti spinach extract and
green giti spinach extract are significantly different from the positive control
quercetin. Antioxidant activity of quercetin is categorized strong antioxidant
activity, while the antioxidant activity of red giti spinach extract and green giti
spinach extract is categorized as weak antioxidant activity.

Keywords

Amaranthus tricolor L Flavonoid Antioxidant DPPH

Article Details

References

  1. Anonim, (2008). Farmakope Herbal Indonesia Edisi Pertama. Departemen Kesehatan Republik Indonesia, Jakarta.
  2. Chang, C.C., Yang, M.H., Wen, H., Chern, J., (2002). Estimation of total flavonoid content in propolis by two complementarycolometric methods. Journal of Food and Drug Analysis. 10(3): 178-182.
  3. Cheng, S.H., Khoo, H.E., Ismail, A., Abdul-Hamid, A., and Barakatun-Nisak, M.Y., (2016). Influence Of Extraction Solvents On Cosmos Caudatus Leaf Antioxidant Properties. Iranian Journal of Science and Technology. 40.
  4. Dehpour, A.A., Ebrahimzadeh, M.A., Fazel, N. S., and Mohammad, N.S., (2009). Antioksidant Activity of Methanol Extract of Ferula Assafoetida and Its Essential Oil Composition. Grasas Aceite. 60(4), 405-412.
  5. Ipandi, I., Triyasmono, L., Prayitno, B., (2016). Penentuan Kadar Flavonoid Total dan Aktivitas Antioksidan Ekstrak Etanol Daun Kajajahi (Leukcosyke capitellata Wedd.). Pharmasience. 3(1): 93-100.
  6. Kelly, G.S., (2011). Quersetin, Alternative Medicine Review. 16(2):172-194
  7. Lobo, V., Patil, A., Phatak, A., Chandra, N., (2010). Free Radicals, Antioxidants and Functional Foods : Impact On Human Health. Pharmacogn Rev. 4(8): 118-126.
  8. Nana, F.W., Hilou, A., Millogo, J.F., Nacoulma, O.G., (2012). Phytochemical Composition, Antioxidant and Xanthine Oxidase Inhibitory Activities of Amaranthus cruentus L. and Amaranthus hybridus L. Extracts. Pharmaceuticals. 5(6), 613-628.
  9. Nurhaeni, F., Lestari, T., Wahyuono, S., Rohman, A., (2014). Aktivitas Antioksidan Ekstrak Etanolik Berbagai Jenis Sayuran Serta Penentuan Kandungan Fenolik dan Kandungan Totalnya. Media Farmasi. 11(2): 167-178.
  10. Panche, A.N., Diwan, A.D., Chandra, S.R., (2016). Flavonoids: an overview. Journal of Nutritional Science. 5(47): 1-15.
  11. Pulipati, S., Babu, P.S., Naveena, U., Parveen, S.K., Nausheen, S.K., Sai, M., (2017). Determination of Total Phenolic, Tannin, Flavonoid Contents and Evaluation of Antioxidant Property of Amaranthus tricolor (L). International Journal of Pharmacognosy and Phytochemical Research. 9(6); 814-819.
  12. Putrawan, Bahriul, Nurdin, Rahman., Anang, Wahid, (2014). Uji Aktivitas Antioksidan Ekstrak Daun Salam (Syzygium Polyanthum) dengan Menggunanakan 1,1-Difenil-2-Pikrilhidrazil. J. Akad. Kim. 3(3): 143-149, ISSN 2302-6030.
  13. Renee, L.B., Kubola, J., Siriamornpun, S., Herald, T.J., and Shi, Y.C., (2014). Wheat bran particles size influence on phytochemical extracbility and antioxidant properties. Jurnal Food Chemistry. 152: 483-490.
  14. Rodrigues Magalhaes, A.L.K., Zorzi, K., (2008). Residue from common bean (Phaseolus vulgaris L.) processing in the rations for milking cows: intake, digestibility, milk production and composition and feeding efficiency. Rev. Bras. Zoot. 37 (3): 529-53
  15. Thomas, A.N.S., (2008). Tanaman Obat Tradisional. Penerbit Kanisius. Yogyakarta.
  16. Salamah, N., Widyasari, E., (2015). Aktivitas Antioksidan Ekstrak Metanol Daun Kelengkeng (Euphoria longan (L) Steud.) dengan Metode Penangkapan Radikal 2,2'-Difenil-1-Pikrilhidrazil. Pharmaciana. 5(1): 25-34.